Periplocin Induces Apoptosis of Pancreatic Cancer Cells through Autophagy via the AMPK/mTOR Pathway

Figure 4

Periplocin-induced autophagy is dependent on AMPK/MTOR signaling. (a, b) After 24 h of periplocin treatment (125 nM, 250 nM). The expression of AMPK/MTOR signaling pathway proteins detected by Western blotting analysis. (c, d) Expression of LC3BII/I of CFPAC1 and PANC-1 cells after treatment with 125 nM periplocin and/or 5 μM CC detected by Western blotting analysis. (e) Expression of LC3B of CFPAC1 and PANC-1 cells after treatment with 125 nM periplocin and/or 5 μM CC detected by immunofluorescence. The data represent the mean ± SE, with , , compared between two groups and , and compared with the group treated with Periplocin and CC (n = 3).

(a)

(b)

(c)

(d)

(e)

(f)