miR-193a-5p Enhances the Radioresistance of Pancreatic Cancer Cells by Targeting ZFP57 and Activating the Wnt Pathway

Figure 1

The correlation between ZFP57 expression and radioresistance of PCC was assessed. 10 pancreatic cancer cases achieving CR+PR and 10 patients achieving SD+PD were recruited. Cancerous tissues from these cases were exposed to X-ray irradiation. (a) ZFP57 expression in tissues of pancreatic cancer before irradiation as detected by RT-qPCR. (b) ZFP57 expression in pancreatic cancer tissues before irradiation as detected by WB. (c) Survival curve of TCGA PAAD cases achieving CR+PR and SD+PD after radiotherapy based on ZFP57 expression. BxPC3 cells (specifically named parental BxPC3-P cells in the present study) were exposed to repeated X-ray irradiations to induce the radioresistant cell subtype BxPC3-RR. (d) Survival fractions of BxPC3-P and BxPC3-RR cells after exposed to 0, 2, 4, and 8 Gy IR⁺ and IR^- as detected by the colony formation assay. (e) ZFP57 expressions in BxPC3-P and BxPC3-RR cells after exposed to 8 Gy IR⁺ and IR^- as detected by qPCR. (f) ZFP57 expression in BxPC3-P and BxPC3-RR cells after exposed to 8 Gy IR⁺ and IR^- as detected by WB. and .

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