miR-193a-5p Enhances the Radioresistance of Pancreatic Cancer Cells by Targeting ZFP57 and Activating the Wnt Pathway

<div>The radiosensitivity of BxPC3-RR cells with induced ZFP57 overexpression was evaluated by detecting cancer cell proliferation and viability after transfection with pc DNA 3.0 and exposure to different irradiation doses. (a) The ZFP57 expression was determined in BxPC3-RR cells transfected with pc DNA 3.0 ZFP57 and the corresponding control. The BxPC3-P cells transfected with sh-ZFP57-2 and the corresponding control were exposed to 8 Gy irradiation. Then, (b) the survival fraction of the BxPC3-RR cells was estimated by colony formation assay. (c) CCK-8 assay was performed to assess the BxPC3-RR cell viability. (d) WB assay was performed to determine the expressions of cyclin D1 and CDK4 (cell cycle-related proteins); Bax, Bcl-2, and cleaved caspase-3 (apoptosis-related proteins); <i>γ</i>-H2AX (DNA repair-related protein); and (e) <i>β</i>-catenin and c-Myc expressions. <span class="nowrap"><svg height="10.7395pt" id="M53" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.0498162 -10.1025 68.3436 10.7395" width="68.3436pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 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Journal of Oncology

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Figure 3

Figure 3: miR-193a-5p Enhances the Radioresistance of Pancreatic Cancer Cells by Targeting ZFP57 and Activating the Wnt Pathway 