miR-193a-5p Enhances the Radioresistance of Pancreatic Cancer Cells by Targeting ZFP57 and Activating the Wnt Pathway

Figure 6

Upstream miRNAs regulating ZFP57 were analyzed. (a) miRWalk database was used in conjunction with the GEO database to predict miRNAs targeting ZFP57. (b) RNA pull-down assay was performed on BxPC3-RR cells. The enriched miRNA was determined by qPCR. (c) The binding site of ZFP and miR-193a-5p was predicted by bioinformatics tools. The prediction was further verified by the luciferase reporter gene assay. The dual-luciferase reporter gene assay was performed on the transfected BxPC3-RR cells. (d) In BxPC3-RR cells, miR-193a-5p expression was silenced. mRNA and protein expressions of ZFP57 were determined by qPCR and WB assay, respectively. (e) miR-193a-5p overexpression was induced in BxPC3-P cells. The protein and miRNA levels of ZFP57 were determined by WB and qPCR. (f) miR-193a-5p levels in pancreatic cancer tissues were determined by qPCR. (g) Pancreatic cancer cells were exposed to IR, and the miR-193a-5p expression was detected by qPCR subsequently. and .

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