The lncRNA NEAT1 Inhibits miRNA-216b and Promotes Colorectal Cancer Progression by Indirectly Activating YY1

Figure 2

Upregulation of NEAT1 in the CRC tissues and cells. (a) NEAT1 is overexpressed in the CRC tissues (n = 32) compared to the normal tissues (n = 32) by qRT-PCR. (b) Detecting NEAT1 expression in CRC cells compared to normal cells by qRT-PCR. (c) Exploring NEAT1 expression in different histological differentiation (well: 25 and poor: 32) of CRC tissues by qRT-PCR. (d) Discovering NEAT1 expression in different clinical stages (stage (i): 25, stage II: 21, stage III: 24, and stage IV: 31) of CRC tissues by qRT-PCR. (e) NEAT1 detection in CRC with metastasis or nonmetastasis tissues (positive: 64 and negative: 79) by qRT-PCR. (f) Upregulation of NEAT1 in CRC with distant metastasis tissues (positive: 19 and negative: 21) determined by qRT-PCR. (g) Upregulation of NEAT1 in CRC with distant metastasis tissues (positive: 13 and negative: 11) detected by qRT-PCR. (h) (i) The Kaplan–Meier survival curve demonstrated that upregulation of NEAT1 in patients with CRC indicated poor prognosis in the overall survival (H) and progress in-free survival (I). and . (j) ROC curve analysis of the NEAT1 expression in colorectal tissues. (k) Different expressions of NEAT1 is related to different histological differentiation, shown by HE staining.

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