The lncRNA NEAT1 Inhibits miRNA-216b and Promotes Colorectal Cancer Progression by Indirectly Activating YY1

Figure 4

NEAT1-inactivating miR-216b to promote cell progression in CRC. (a) Online bioinformatics tool to analyze the binding site of NEAT1 and miR-216b. (b) Detecting expression of miR-216b mRNA expression by qRT-PCR in HCT-116 cell with NEAT1 silenced or control. (c) Exploring miR-216b expression in CRC tissues and normal tissues by qRT-PCR. (d) Detection of luciferase activity in cell lysates in HCT-116 cells cotransfected with wild-type or mutant NEAT1 plasmid and miR-216b mimic or miR-216b inhibitor. (e) Determining NEAT1 expression in HCT-116 cell transfected with miR-216b mimic or miR-216b inhibitor. (f) Discovering miR-216b expression in HCT-116 cell transfected with NEAT1 silence or control. (g) Detection of miR-216b by qRT-PCR in the sample pulled down by the biotinylated NEAT1 probe. (h) Exploration of cell migration and invasion by transwell assays in HCT-116 and SW620 cells transfected with miR-216b mimic, or the effect of miR-216b on proliferation in HCT-116 and SW620 cells cotransfected with NEAT1-silencing plasmid and miR-216b mimic or miR-216b inhibitor via CCK-8 assay. , , compared with the NC group.

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