The lncRNA NEAT1 Inhibits miRNA-216b and Promotes Colorectal Cancer Progression by Indirectly Activating YY1

Figure 5

YY1 targeted by miR-216b and positively regulated by NEAT1 in CRC cells. (a) The 3′-UTR of YY1 contained miR-216b binding site by miRDB tool. (b) YY1 overexpressed in CRC tissues compared with normal tissues. (c) The luciferase activity was measured in HCT-116 cells transfected with miR-216b mimics or miR-216b inhibitor for 48 h. (d) The expression of YY1 mRNA and protein were detected by qRT-PCR and western blot assays in HCT-116 and SW620 cells with NEAT1 silence. (e) The expression of YY1 mRNA and protein were detected by qRT-PCR and western blot assays in HCT-116 and SW620 cells transfected with miR-216b mimic. (f) The expression of YY1 mRNA and protein were detected by qRT-PCR and western blot assays in HCT-116 and SW620 cells transfected with NEAT1 silence or cotransfected with miR-216b mimic. (g) Cell invasion and migration were tested by the transwell assay in HCT-116 and SW620 cells with NEAT1 silence. (h) The expression of YY1 protein in CRC tissues, liver metastatic tissues, and normal tissues was verified by IHC. , , compared with the NC group.

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