Long Intergenic Noncoding RNA 00641 Promotes Growth and Invasion of Colorectal Cancer through Regulating miR-450b-5p/GOLPH3 Axis

Figure 3

Cytoplasmic LINC00641 targeted miR-450b-5p in CRC cells (a) LINC00641 mainly existed in the cytoplasm, which was determined by subcellular fraction assay. (b) Venn diagram illustrating the overlap of LINC00641-targeting miRNAs in databases starBase and LncBase and downregulated miRNAs in GSE81581 dataset from GEO database. (c) At the complementary locations for the seed region in miR-450b-5p, mutations were introduced into the LINC00641 sequence. (d) The activities of the WT and mutant of LINC00641 were tested by luciferase reporter assay when has-miR-450b-5p was upregulated. (e) qRT-PCR analysis of the mRNA level of LINC00641 in SW480-NC, SW480- miR-450b-5p, HCT-116-NC, and HCT-116- miR-450b-5p cell lines. (f) qRT-PCR analysis of the level of miR-450b-5p in SW480-shNC, SW480-sh LINC00641, HCT-116-shNC, and HCT-116-shLINC00641 cell lines. (g) The correlation between miR-450b-5p and LINC00641 expression in CRC tissues. Compared with shNC group or mimic NC group, , , ns .

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