Long Intergenic Noncoding RNA 00641 Promotes Growth and Invasion of Colorectal Cancer through Regulating miR-450b-5p/GOLPH3 Axis

Figure 5

GOLPH3 was the downstream miR-450b-5p target. (a) Venn diagram illustrated the overlap of mRNAs in databases TargetScan, starBase, miRDB, and miRTarBase and predicted the putative downstream targets of miR-450b-5p. (b) GOLPH3’s 3′UTR sequence was mutated at the corresponding locations for the seed region in miR-450b-5p. (c) The effects of miR-450b-5p expression on the activities of the WT and mutant 3′UTR of GOLPH3 in SW480 and HCT-116 cells were shown in a bar chart of Luciferase reporter assay analysis. (d) qRT-PCR analysis of the mRNA level of GOLPH3 in SW480-NC, SW480-miR-450b-5p, HCT-116-NC, and HCT-116- miR-450b-5p cell lines. (e) Western blot analysis showed the level of GOLPH3 proteins in SW480-NC, SW480-miR-450b-5p, HTC-116-NC, and HCT-116-miR-450b-5p cell lines. (f) The correlation between miR-450b-5p and GOLPH3 expression in CRC tissues was analyzed by starBase. (g) qRT-PCR analysis of the mRNA level of GOLPH3 in SW480-shNC, SW480-sh LINC00641, HTC-116-shNC, and HCT-116- shLINC00641 cell lines. (h) Representative image of western blotting showed the level of GOLPH3 proteins in SW480-shNC/shLINC00641 and HCT-116-shNC/shLINC00641 cell lines. (i) starBase analyzed the correlation between LINC00641 and GOLPH3 expression in CRC tissues. Compared with mimic NC group, , , ns .

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