MiR-205-5p Functions as a Tumor Suppressor in Gastric Cancer Cells through Downregulating FAM84B

Figure 4

MiR-205-5p bound to FAM84B and inhibited its expression. (a) The binding sites between miR-205-5p and FAM84B 3′ untranslated region (3′ UTR) were predicted on the Starbase website (http://starbase.sysu.edu.cn/index.php). (b) A dual-luciferase reporter assay was performed to confirm the direct interaction between miR-205-5p and FAM84B 3′ UTR. (c) NC mimic and miR-205-5p mimic were transfected into AGS and SGC-7901 cells by using Lipofectamine 2000. The expression of FAM84B mRNA was detected by qRT-PCR. (d) NC mimic and miR-205-5p mimic were transfected into AGS and SGC-7901 cells by using Lipofectamine 2000. The expression of the FAM84B protein was detected by western blot. <0.01 and <0.001 vs. NC mimic. All experiments were performed in triplicate and repeated at least three times.

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