MiR-205-5p Functions as a Tumor Suppressor in Gastric Cancer Cells through Downregulating FAM84B

Figure 5

FAM84B overexpression reversed antitumor effects of miR-205-5p in GC. The experiments were performed in three groups: (1) NC mimic + Lv-NC; (2) miR-205-5p mimic + Lv-NC; and (3) miR-205-5p mimic + Lv-FAM84B. (a) The recombinant lentivirus vectors that contained FAM84B full sequence or NC sequence were transfected into AGS and SGC-7901 cells, and FAM84B expression was detected by qRT-PCR. <0.001 vs. Lv-NC (b) Cell viability of transfected AGS and SGC-7901 cells was detected by using the CCK-8 assay. (c) Colony formation of transfected AGS and SGC-7901 cells was detected. (d) Cell apoptosis of transfected AGS and SGC-7901 cells was detected by flow cytometry. (e) Transwell assay investigated the migration and invasion activities of transfected cells. <0.01 and <0.001 vs. NC mimic; <0.01 vs. MiR-205-5p mimic + Lv-NC. All experiments were performed in triplicate and repeated at least three times.

(a)

(b)

(c)

(d)

(e)