M2 Tumor Associate Macrophage- (TAM-) Derived lncRNA HISLA Promotes EMT Potential in Bladder Cancer

Figure 1

Identification of M2-like TAMs from THP-1 and TAM-derived exosomes. (a, b) M0 THP-1 cells were incubated with IL-4 and IL-13 to promote M2-TAM polarization, and the (a) mRNA levels of M2-TAM markers IL-10, CCL-18, CD206, and CD163 were detected by qRT-PCR, and the (b) protein levels of Clever-1 and Arg-1 (also M2-TAM markers) were examined by western blot. (c) Representative electron micrograph of exosomes derived from TAMs. Scale bar, 100 nm. (d) The diameter of exosomes derived from the medium of M0 or TAMs. (e) Western blot analysis of the expression of CD9, CD81, and HSP70 in the cells and exosomes. Data shown are representative images or expressed as the . compared to the control.

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