M2 Tumor Associate Macrophage- (TAM-) Derived lncRNA HISLA Promotes EMT Potential in Bladder Cancer

Figure 4

Silencing of lncRNA-HISLA in TAM-exo suppresses migration, invasion, and EMT process of bladder cells. (a) M2-TAMs were treated with si-Control or si-HISLA siRNAs, and the efficiency of silencing was confirmed by qRT-PCR. (b, c) Wound healing assay was performed to detect the effect of silencing HISLA in TAM-exo on migration of T24 and HTB-1 cells. (d, e) Transwell assay (without matrigel) was used to illustrate the effect of silencing HISLA in TAM-exo on cell migration. (f, g) Transwell assay (with matrigel) was used to illustrate the effect of silencing HISLA in TAM-exo on cell invasion. (h) EMT markers such as E-cadherin, N-cadherin, Vimentin, and VEGF were detected by western blot assay after TAM-exo treatment in T24 and HTB-1 cells. (i) Representative images of lung metastasis in metastatic experiments. (j) Representative photos of tumor tissues in xenograft models. Data shown are representative images or expressed as the . compared to the control.

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