M2 Tumor Associate Macrophage- (TAM-) Derived lncRNA HISLA Promotes EMT Potential in Bladder Cancer

Figure 6

Exosomal lncRNA-HISLA derived from TAMs stabilized β-catenin in BC cells through preventing interaction between GSK3β and β-catenin. (a, b) After treatment with protein synthesis inhibitor CHX for indicated times, the protein levels of β-catenin in HISLA silenced TAM-exosome-treated T24 cells were examined by western blot. (c) Interaction between GSK3β and β-catenin was detected by immunoprecipitation in HISLA overexpressed T24 or HTB-1 cells. (d) Interaction between GSK3β and β-catenin was detected by immunoprecipitation in HISLA silenced T24 or HTB-1 cells with the existence of proteasome inhibitor mg132. (e) Immunofluorescence was used to analyze the interaction between GSK3β and β-catenin in HISLA overexpressed T24 cells. (f) si-HISLA and β-catenin were cotransfected into T24 cells, and the protein levels of E-cadherin, N-cadherin, and Vimentin were detected by western blot. Data shown are representative images or expressed as the . compared to the control.

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