Deferoxamine Inhibits Acute Lymphoblastic Leukemia Progression through Repression of ROS/HIF-1α, Wnt/β-Catenin, and p38MAPK/ERK Pathways

Figure 2

The effect of DFO on cell proliferative activity and cell cycle of Jurkat and NALM-6 cells. (A, B) Proliferation of cells treated with DFO (100 µmol/L), or DFO (100 µmol/L) and Dex (20 µg/mL) in combination was measured by CCK-8 assays after culturing for indicated time. (C, D) ALL cells were treated with DFO (100 µmol/L) or DFO (100 µmol/L) and Dex (20 µg/mL) in combination for 48 h. Western blot analyses for expression of Ki67 in ALL cells. (E, F) At 48 h after indicated treatments (DFO (100 µmol/L), or DFO (100 µmol/L) and Dex (20 µg/mL)), cell cycle distribution of Jurkat and NALM-6 cells was determined by the flow cytometry assay. Data are presented as mean ± SD, n = 3. .

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