Long Noncoding RNA RMRP Contributes to Paclitaxel Sensitivity of Ovarian Cancer by Regulating miR-580-3p/MICU1 Signaling

Figure 4

RMRP is able to sponge miR-580-3p. (a) The binding site prediction of RMRP and miR-580-3p in ENCORI database. (b–d) The HeyA-8/PTX and SKOV3/PTX cells were treated with miR-580-3p mimic. (b) The miR-580-3p expression was analyzed by qPCR. (c, d) The luciferase activity was detected by dual luciferase reporter assays. (e, f) The HeyA-8/PTX and SKOV3/PTX cells were treated with RMRP siRNA. The miR-580-3p expression was analyzed by qPCR. (g) The direct interaction of RMRP and miR-580-3p was analyzed by RNA pull down. (h) The miR-580-3p expression was measured in HeyA-8 and SKOV3 cells and PTX-resistant HeyA-8 (HeyA-8/PTX) and SKOV3 (SKOV3/PTX) cells by qPCR. shcontrol, control shRNA; shRMRP, RMRP shRNA. Mean ± SD, .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)