Long Noncoding RNA RMRP Contributes to Paclitaxel Sensitivity of Ovarian Cancer by Regulating miR-580-3p/MICU1 Signaling

Figure 5

MiR-580-3p is able to target MICU1 in ovarian cancer cells. (a) The binding site prediction of MICU1 and miR-580-3p in ENCORI database. (b, c) The HeyA-8/PTX and SKOV3/PTX cells were treated with miR-580-3p mimic. (b) The luciferase activity was detected by dual luciferase reporter assays. (c) The expression of MICU1 was detected by qPCR. (d) The HeyA-8/PTX and SKOV3/PTX cells were co-treated with RMRP shRNA and miR-580-3p inhibitor. The expression of MICU1 was detected by qPCR. (e) The MICU1 expression was measured in HeyA-8 and SKOV3 cells and PTX-resistant HeyA-8 (HeyA-8/PTX) and SKOV3 (SKOV3/PTX) cells by qPCR. (f) The MICU1 and miR-580-3p expression was analyzed in ovarian cancer patient tissues (n = 36) and the normal cases (n = 36) by qPCR. (g) The MICU1 and miR-580-3p expression was detected in PTX-resistant (n = 16) and PTX sensitive (n = 16) ovarian cancer patient tissues by qPCR. Mean ± SD, .

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