Research Article
MACC1 Promotes the Progression and Is a Novel Biomarker for Predicting Immunotherapy Response in Colorectal Cancer
Figure 2
The expression of MACC1 in SW480 and HCT116 cell lines. (a) RT-qPCR validated the MACC1 overexpression and interference efficiency. (b) Cell viability was determined by CCK-8 assay after the overexpression of or interfered with MACC1. (c) Western blot validated the MACC1 overexpression and interference efficiency. (d) EdU detected proliferation after interference with MACC1. (e) Cell migration was determined by a scratch assay after MACC1 interference. (f) Cell invasion was detected after interference by the MACC1 invasion assay. (g) Clone formation assay validated the interference efficiency of MACC1. (h) Cell proliferation markers and statistics were measured by WB after interference with MACC1. The results are shown as means ± SEM; , , and .
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