GABPB1-AS1 Promotes the Development of Osteosarcoma by Targeting SP1 and Activating the Wnt/β-Catenin Pathway

Figure 6

GABPB1-AS1 mediated osteosarcoma cells through the SP1/Wnt/β-catenin signaling pathway. (a) Western blot to detect the protein levels of SP1, Wnt, β-catenin, c-Myc, and SOX2 in osteosarcoma cells when GABPB1-AS1 is silent or not. (b) Colony formation to evaluate the rescue effect of LiCl treatment on the proliferation of GABPB1-AS1-silenced osteosarcoma cells. (c) Overexpression of lncRNA GABPB1-AS1 can upregulate the expression of SP1. (d) Wound healing test to evaluate the rescue effect of LiCl treatment on the migration of GABPB1-AS1 silenced osteosarcoma cells, scale bar = 200 μm. (e) Transwell evaluates the rescue effect of LiCl treatment on the invasion of GABPB1-AS1 silenced osteosarcoma cells, scale bar = 100 μm. (f) qRT-PCR detects the level of EMT biomarker E-cadherin in different treated cells. (g) qRT-PCR detects the level of EMT biomarker N-cadherin in different treatment cells. (h) qRT-PCR detects the level of EMT biomarker Slug in different treatment cells. (i) qRT-PCR detects the level of EMT biomarker Twist in different treated cells. , , and .

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