Upregulation of PNCK Promotes Metastasis and Angiogenesis via Activating NF-κB/VEGF Pathway in Nasopharyngeal Carcinoma

Figure 4

The activity of the NF-κB/VEGF signaling pathway is upregulated by PNCK overexpression in NPC cells. (a) Dot plot of Ingenuity Pathway Analysis (IPA) shows significant changes in NF-κB and VEGF signaling pathways. (b) GSEA shows negative regulation of NF-κB transcription factor activity enriched in the PNCK-low expression phenotype. (c) WB was used to investigate the P65, p-P65, p-IκB, GAPDH, and Histone-H3. The correlation between PNCK and VEGFA in (d) internal cohort and (e) GSE102349 cohort. (f, g) The expression of VEGFA levels in NPC cells was evaluated by PCR and WB.

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