IMP4 Silencing Inhibits the Malignancy of Lung Adenocarcinoma via ERK Pathway

Figure 2

IMP4 silencing inhibited proliferation, promoted apoptosis, and induced cell cycle arrest in LUAD cells. After treatment of A549 and H1299 cells with si-IMP4-1 and si-IMP4-2, IMP4 levels were determined with qRT-PCR (a); proliferation was examined applying CKK-8 (b) and EdU analysis (c); apoptosis was detected using Annexin-V/PI double staining kit (d); the expression of Bcl-2, Bax, and PARP was measured using Western-blotting (e); cell cycle was evaluated applying cell cycle and apoptosis analysis kit (f). Protein levels of cyclin D1, P21, and P53 in A549 and H1299 cells (g). , .

(a)

(b)

(c)

(d)

(e)

(f)

(g)