IMP4 Silencing Inhibits the Malignancy of Lung Adenocarcinoma via ERK Pathway

Figure 7

E2F4 up-regulated IMP4 and promoted the progression of LUAD. (a) Venn chart identified E2F4 via analysing the Animal TFDB, hTFtarget, and Chipbase among the genes positively associated with IMP4 expression in TCGA-LUAD. (b) TCGA-LUAD database verified that IMP4 was positively related to E2F4 expression. (c) The expression of E2F4 in LUAD was analysed using the UALCAN based on TCGA database. (d) The survival curves of LUAD patients from GEPIA database. (e) Luciferase activities were evaluated via performing dual luciferase reporter assay. After pcDNA3.1-E2F4 vector was transfected into A549 cells, the mRNA levels of IMP4 (f) and E2F4 (g) were determined using qRT-PCR. After pcDNA3.1-E2F4 vector and si-IMP4-2 co-transfection, A549 cell proliferation was analysed using the EdU assay (h); apoptosis was assessed using FITC-Annexin V/PI apoptosis detection kit (i); the cell cycle was evaluated using cell cycle and apoptosis analysis kit (j); invasion was examined using the transwell assay (k). , , .

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