CircFBXW7 Inhibits Proliferation, Migration, and Invasion of Nonsmall Cell Lung Cancer Cells by Regulating miR-492

Figure 2

Overexpression of circFBXW7 reduced cell proliferation, migration, and invasion. (a) circFBXW7 downregulated in NSCLC cells, including HCC827, PC9, NCI-H23, and NCI-H1299 cells. qRT-PCR was used to test the expression of circFBXW7. (b) circFBXW7 expression upregulated by oe-circFBXW7 transfection for 48 h in NCI-H23 and NCI-H1299 cells. ((c) and (d)) Overexpression of circFBXW7 decreased proliferation in NCI-H1299 and NCI-H23 cells; CCK-8 was used to assess cell proliferation. ((e) and (f)) Overexpression of circFBXW7 reduced the migration and invasion ability of NCI-H1299 and NCI-H23 cells; Transwell assays were used to assess cell migration and invasion (40x). FBXW7, F-box and WD repeat domain containing 7; oe-NC, circFBXW7 overexpression negative control; oe-circFBXW7, circFBXW7 overexpression plasmids; qRT-PCR, real-time quantitative polymerase chain reaction; CCK-8, cell counting kit-8; .

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