CircFBXW7 Inhibits Proliferation, Migration, and Invasion of Nonsmall Cell Lung Cancer Cells by Regulating miR-492

Figure 4

CircFBXW7 reduced cell proliferation, migration, and invasion by regulating miR-492. (a) The expression of miR-492 was downregulated by circFBXW7 overexpression, whereas this effect was reversed by miR-492 mimics in NCI-H23 and NCI-H1299 cells; the expression of miR-492 was determined by qRT-PCR after transfection for 48 h. ((b) and (c)) Proliferation was reduced by circFBXW7 overexpression, whereas this effect was reversed by miR-492 mimics in NCI-H23 and NCI-H1299 cells; the proliferation of NCI-H23 and NCI-H1299 cells was detected at 24, 48, and 72 h by CCK-8 assay after transfection for 48 h. ((d) and (e)) migration and invasion were decreased by circFBXW7 overexpression, whereas this effect was reversed by miR-492 mimics in NCI-H23 and NCI-H1299 cells. Migration and invasion were detected via Transwell assay after transfection for 48 h (40x). FBXW7, F-box and WD repeat domain containing 7; oe-NC, circFBXW7 overexpression negative control; oe-circFBXW7, circFBXW7 overexpression plasmids; qRT-PCR: real-time quantitative polymerase chain reaction; CCK-8: cell counting kit-8; vs. the NC group, vs. the oe-circFBXW7 group.

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