Trained Immunity of IL-12-, IL-15-, and IL-18-Induced CD₃+CD₅₆+ NKT-Like Cells

Figure 1

IL-12/15/18-induced CD₃+CD₅₆+ NKT-like cells increase IFN-γ release. (a) Overview of experimental design. CD₃+CD₅₆+NKT-like cells were preactivated with rhIL-12 (10 ng/mL), IL-15 (1 ng/ml), IL-18 (50 ng/ml), or control (1 ng/ml of IL-15 alone) for 16 hours. Flow cytometry analysis was performed at indicated time point after preactivation. (b) The gating strategy for CD₃+CD₅₆+NKT-like cells and percentages of IFN-γ+ cell populations by flow cytometry plots. Percentages of cells and enhanced IFN-γ production restimulated with same IL-12/15/18 on day 7. n = 8, none, (error bars, mean ± SEM). (c) Expression of Ki67 restimulated with same IL-12/15/18 on day 7. TNF-α production restimulated with same IL-12/15/18 on day 7. n = 8, none, (error bars, mean ± SEM).

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