Trained Immunity of IL-12-, IL-15-, and IL-18-Induced CD<sub>3</sub>+CD<sub>56</sub>+ NKT-Like Cells

<div>IL-12/15/18 preactivated CD3+CD56+ NKT-like cells enhance the intrinsic potential and show an increase in IFN-<i>γ</i> release when stimulated by K562 tumor cells. (a) Percentages of IFN-<i>γ</i>+ TNF-<i>α</i>+ and Ki67+ cell populations by flow cytometry plots. 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602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,55.606,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,61.846,0)"><path d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg> (error bars, mean ± SEM).(b) The gating strategy for percentages of IFN-<i>γ</i>+ cell populations cocultured with K652 on Day 7; an overlaid histogram shows increased expression of Ki67 and enhanced level of GzmB. <i>n</i> = 8. (c) Overlaid histograms show expression of IFN-<i>γ</i>, Ki67, and GzmB cocultured with K562 on day 7 by different concentrations of cytokines preactivating.</div>

Journal of Oncology

fig2

Figure 2

Figure 2: Trained Immunity of IL-12-, IL-15-, and IL-18-Induced CD<sub>3</sub>+CD<sub>56</sub>+ NKT-Like Cells 

Journal of Oncology

Trained Immunity of IL-12-, IL-15-, and IL-18-Induced CD3+CD56+ NKT-Like Cells

Figure 2

Trained Immunity of IL-12-, IL-15-, and IL-18-Induced CD₃+CD₅₆+ NKT-Like Cells