The tRNA-Derived Fragment tRF-24-V29K9UV3IU Functions as a miRNA-like RNA to Prevent Gastric Cancer Progression by Inhibiting GPR78 Expression

Figure 2

Knockdown of tRF-24-V29K9UV3IU facilitates growth and metastasis of xenograft tumors in vivo. (a) The efficacy of lentivirus interfering with tRF-24-V29K9UV3IU expression detected by qRT-PCR (). (b) Representative images of subcutaneous tumor formation in mice after LV-tRF-24-V29K9UV3IU-inhibitor-expressing or NC MKN-45 cell inoculation (). (c) Representative images of subcutaneous tumor tissues in mice (). The tumor (d) volume and (e) weight statistics after LV-tRF-24-V29K9UV3IU-inhibitor-expressing or NC MKN-45 cell inoculation (). (f) Representative images for H&E staining in tumors (). Magnification 20x. These results implicated that knockdown of tRF-24-V29K9UV3IU facilitates growth of GC. (g) The expression of tRF-24-V29K9UV3IU in tumor tissues after LV-tRF-24-V29K9UV3IU-inhibitor or NC MKN-45 cell inoculation (). (h) Representative images of H&E staining in lung tissue following LV-tRF-24-V29K9UV3IU-inhibitor-expressing MKN-45 cell injection (). Magnification 20x. (i) mRNA expression of E-cadherin, N-cadherin, and vimentin in tumor tissues after LV-tRF-24-V29K9UV3IU-inhibitor-expressing or NC MKN-45 cell inoculation, detected by qRT-PCR (). ((j) and (k)) Protein expression of vimentin and Snail in tumor tissues after LV-tRF-24-V29K9UV3IU-inhibitor-expressing or NC MKN-45 cell inoculation, detected by western blotting (). These results implicated that knockdown of tRF-24-V29K9UV3IU facilitates lung metastasis of GC. t test, , , .

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