The tRNA-Derived Fragment tRF-24-V29K9UV3IU Functions as a miRNA-like RNA to Prevent Gastric Cancer Progression by Inhibiting GPR78 Expression

Figure 5

tRF-24-V29K9UV3IU inhibits GPR78 expression by binding its 3-UTR in GC cells. (a) Eight DEGs predicted to possibly bind tRF-24-V29K9UV3IU through overlap of DEGs and the Miranda and RNAhybrid database. (b) The regulatory network of tRF-24-V29K9UV3IU. The rounded rectangle represents tRF-24-V29K9UV3IU, the ellipse represents mRNA, the diamond represents a pathway, and the red/green color indicates upregulation and down-regulation, respectively. ((c)–(e)) Expression of GPR78, OLR1, and VEPH1 as verified by qRT-PCR. (f) Putative binding site of tRF-24-V29K9UV3IU on GPR78. (g) The luciferase activity of GPR78 in MKN-45 cells after cotransfection with tRF-24-V29K9UV3IU mimics or NC. (h) An AGO2-RIP assay was conducted to confirm that tRF-24-V29K9UV3IU associates with AGO2. (i) Expression of GPR78 in 19 pairs tumor and normal tissues detected by qRT-PCR. Expression of GPR78 was up-regulated in tumor compared to normal tissues. (j) The expression of GPR78 and tRF-24-V29K9UV3IU was negatively correlated, which was analyzed by Pearson’s correlation in tumors. The above experiments were repeated three times. t test, , , .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)