Synergistic Lethality Effects of Apatinib and Homoharringtonine in Acute Myeloid Leukemia

Figure 5

In AML cell lines, Apatinib and HHT decreased mitochondrial membrane potential and altered p-VEGFR-2, PI3K/Akt pathway associated proteins. (a, b) MV4-11 and MOLM-13 cells with loss of mitochondrial membrane potential. (c) MOLM-13 cells were exposed to the indicated concentrations of Apatinib ± HHT for 24 h and 48 h after which western blot analysis was shown to monitor the expression of p-VEGFR-2, PI3K/Akt pathway associated proteins, anti-apoptotic proteins BCL-2 and MCL-1. (d) pharmacologic targeting VEGFR-2 and MCL-1 induces mitochondrial dysfunction, decreased intracellular MMP levels, and apoptosis in AML.

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