The HBV Specially-Related Long Noncoding RNA HBV-SRL Involved in the Pathogenesis of Hepatocellular Carcinoma

Figure 2

Investigation of HBV-SRL coding capacity. (a) Diagram of HBV-SRL transcript. Potential open reading frame (ORF) ranged from 830 to 1333. (b) Bioinformatic analysis of coding capacity of HBV-SRL using CPC, CNCI, PFAM, and CPAT. CPC, coding potential calculator; CNCI, coding-noncoding index; CPAT, coding potential assessment tool. Transcript with CPC score < 0, CNCI score < 0, PFAM = nonsignificant, and CPAT <0.364 was a potential lncRNA. (c) Real-time PCR analysis of HBV-SRL (A) and western blot analysis of His expression (B) in CSQT-2 cells transfected with plasmid pAdeno-His (Control), pAdeno-HBV-SRL-His (HBV-SRL-His), or pSMYD4-His (SMYD4- His). (d) Real-time PCR analysis of HBV-SRL (A) and western blot analysis by anti-HBV-SRLP antibody (B) using CSQT-2 cells transfected with plasmid pAdeno-His (Control), pAdeno-HBV-SRL-His (HBV-SRL-His), or siRNAs targeting HBV-SRL (si-HBV-SRL). The error bars represent the standard deviation (SD) of data obtained in at least three independent experiments, .

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