The HBV Specially-Related Long Noncoding RNA HBV-SRL Involved in the Pathogenesis of Hepatocellular Carcinoma

Figure 3

Regulation of NF-kB2 and tumor cells growth by HBV-SRL. (a) Luciferase analysis using CSQT-2 cells transfected with pAdeno-HBV-SRL-His and pGL3-NF-κB2 promoter-wild type (pGL3-WT), pGL3-NF-κB2 promoter-mutant (pGL3-MUT), or pGL3-enhancer (pGL3). (b) Real-time PCR (A) and western blot (B) analysis of NF-κB2 in CSQT-2 cells transfected with plasmid pAdeno-His (Control) and pAdeno-HBV-SRL-His (HBV-SRL-His). (c) Real-time PCR (A) and western blot (B) analysis of NF-κB2 in CSQT-2 cells transfected with siRNAs targeting HBV-SRL (si-HBV-SRL) and NC control (NC). (d) Cell cycle (A) or cell growth (B), CSQT-2 cells transfected with siRNAs targeting HBV-SRL (si-HBV-SRL) and NC control (NC) analyzed by flow cytometry and CCK-8, respectively. (e) Image (upper) and tumor growth curve (lower) of tumor tissues from mice treated with siRNAs targeting HBV-SRL (si-HBV-SRL) or negative control (NC). N = 6 each group. The error bars represent the standard deviation (SD) of data obtained in at least three independent experiments, .

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