circPOLR1C Promotes the Development of Esophageal Cancer by Adsorbing miR-361-3p and Regulating Cancer Cell Apoptosis and Metastasis

Figure 1

Characteristics of circPOLR1C in EC. (a) The structure of circPOLR1C. (b) The expression of circPOLR1C in EC tissues and adjacent tissues was detected by quantitative real-time polymerase chain reaction (qRT-PCR) (n = 46). (c) The expression of circPOLR1C in EC cells and normal human esophageal cells was quantified by qRT-PCR. (d) Random hexamer primers and oligo(dT) 18 primers were used to analyze the expressions of circPOLR1C and linear POLR1C by qRT-PCR. (e) The expression stability of circPOLR1C was investigated by the RNase R experiment. (f) 0 h, 4 h, 8 h, 12 h, and 24 h after the addition of actinomycin D, the half-lives of circPOLR1C and linear POLR1C were detected by qRT-PCR. (g) The expression of circPOLR1C in EC cells was measured by cytoplasmic nuclei isolation and qRT-PCR. β-Actin and U2 acted as internal references. (h) The expression of circPOLR1C in EC cells was tested by RNA fluorescence in situ hybridization (FISH) under 400 × magnification. All experiments were independently performed in triplicate. ^### vs. adjacent tissue (ANT); vs. Het-1A; ^ ^ ^ vs. random hexamer primers; ^ΔΔΔ vs. RNase R-; ^ξξξ vs. circPOLR1C.

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