Research Article

circPOLR1C Promotes the Development of Esophageal Cancer by Adsorbing miR-361-3p and Regulating Cancer Cell Apoptosis and Metastasis

Figure 2

Effects of sicircPOLR1C on the viability, proliferation, migration, invasion, and apoptosis of EC cells in vitro and EC xenografts and lung metastasis in vivo. (a) The transfection efficiency of sicircPOLR1C was detected by qRT-PCR. GAPDH was applied as the internal reference. (b) The effect of sicircPOLR1C on the viability of EC cells was evaluated by the cell counting kit (CCK)-8 assay. (c, d) The effect of sicircPOLR1C on the proliferation of EC cells was assessed by the cell clone formation assay. (e, f) The effect of sicircPOLR1C on the migration of EC cells was detected by the scratch assay under 100 × magnification. (g, h) The effect of sicircPOLR1C on the invasion of EC cells was determined by the transwell assay under 250 × magnification. (i, j) The effect of sicircPOLR1C on the apoptosis of EC cells was evaluated by flow cytometry. (k, l) The effect of overexpressed circPOLR1C on the growth of EC xenografts, n = 6/group. (m) The effect of overexpressed circPOLR1C on the metastasis of EC was demonstrated by hematoxylin-eosin (HE) staining under 100 × magnification. All experiments were independently conducted in triplicate. vs. siNC; ^ ^ ^ vs. vector.
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