circPOLR1C Promotes the Development of Esophageal Cancer by Adsorbing miR-361-3p and Regulating Cancer Cell Apoptosis and Metastasis

Figure 4

The effects of the miR-361-3p mimic and circPOLR1C on the miR-361-3p expression, viability, proliferation, migration, invasion, and apoptosis of EC cells. (a) The effects of the miR-361-3p mimic and circPOLR1C on miR-361-3p expression were evaluated by qRT-PCR. U6 was exploited as the internal reference. (b) The effects of the miR-361-3p mimic and circPOLR1C on the viability of EC cells were detected by the CCK-8 assay. (c and e) The effects of the miR-361-3p mimic and circPOLR1C on the proliferation of EC cells were evaluated by the cell clone formation assay. (d and f) The effects of the miR-361-3p mimic and circPOLR1C on the migration of EC cells were determined by the scratch assay under 100 × magnification. (g–h) The effects of the miR-361-3p mimic and circPOLR1C on the invasion of EC cells were evaluated by the transwell assay under 250 × magnification. (i, j) The effect of the miR-361-3p mimic and overexpressed circPOLR1C on the apoptosis of EC cells was examined by flow cytometry. All experiments were independently conducted in triplicate. vs. NC + mimic control (MC); ^ ^ ^ vs. NC + miR-361-3p mimic (M); ^### vs. circPOLR1C + MC.

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