circPOLR1C Promotes the Development of Esophageal Cancer by Adsorbing miR-361-3p and Regulating Cancer Cell Apoptosis and Metastasis

Figure 8

Effects of miR-361-3p and circPOLR1C on the expressions of circPOLR1C, miR-361-3p, and Ki-67in vivo and on the number of lung nodules in mice with lung transplantation. (a) The effects of the miR-361-3p mimic and circPOLR1C on the expression of circPOLR1C were evaluated by qRT-PCR. GAPDH was exploited as the internal reference. (b) The effects of the miR-361-3p mimic and circPOLR1C on the expression of miR-361-3p were assessed by qRT-PCR. U6 was utilized as the internal reference. (c) The effects of the miR-361-3p mimic and circPOLR1C on the expression of Ki-67 were analyzed by immunohistochemical staining under 400 × magnification. (d, e) The effects of the miR-361-3p mimic and overexpression of circPOLR1C on the number of lung nodules were evaluated by HE staining under 100 × magnification, n = 6/group. All experiments were independently conducted in triplicate. ; vs. NC + MC;  ^; ^ ^ ^ vs. NC + M; ^### vs. circPOLR1C + MC.

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