Downregulation of S100A9 Reverses Cisplatin-Resistance and Inhibits Proliferation and Migration in Hypopharyngeal Carcinoma

Figure 5

Downregulation of S100A9 reversed the cisplatin sensitivity and inhibited migration and proliferation. Con: a negative control lentivirus transfected group; siS100A9: siS100A9 lentivirus transfected group. (a) A comparison of the fluorescent and white light images after being transfected for 72 hours showed that the transfected efficiency of con and siS100A9 groups were both higher than 80%. (b) Expression of S100A9 protein was determined by western blot after lentivirus transfected and puromycin exposed for 2 weeks. (c) The downregulation of S100A9 attenuated the cisplatin sensitivity in FaDu/DDP by CCK8. (d) The proliferation assay of FaDu/DDP con and siS100A9 cell lines. (e) Number of adherent cells after lentivirus transfected for 72 hours in FaDu/DDP. (f, g) The scratch assay and migration assay showed the migration-inhibition effect of S100A9 downregulation in HPC cisplatin-resistant cells. (h) Expression of the EMT-associated proteins and IL6 protein was determined by Western blot in FaDu/DDP. (i) WB showed IL6 expression levels in FaDu and FaDu/DDP. , , and .

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