Research Article

Exosome-Mediated Transfer of miR-3613-5p Enhances Doxorubicin Resistance by Suppression of PTEN Expression in Breast Cancer Cells

Figure 4

miR-3613-5p targets PTEN. (a) qRT-PCR was used to assess the relative level of PTEN in MCF-7, MCF-7/DOX, MDA-MB-231, and MDA-MB-231/DOX cells. . Data are of 3 independent experiments. (b) Western blotting was used to detect the protein expression of PTEN in MCF-7, MCF-7/DOX, MDA-MB-231, and MDA-MB-231/DOX cells. . Data are mean ± S.D. of 3 independent experiments. (c) Website TargetScan predicted the binding site of PTEN to miR-3613-5p. (d) Dual luciferase reporter assay was performed to detect the luciferase activity in wild-type (WT) and mutant (MUT) of MCF-7/DOX and MDA-MB-231/DOX cells after transfection with NC mimics and miR-3613-5p mimics. . Data are of 3 independent experiments. (e) qRT-PCR was used to assess the relative levels of miR-3613-5p and PTEN in MCF-7/DOX and MDA-MB-231/DOX cells. . Data are of 3 independent experiments. (f) Western blotting was used to detect the protein expression of PTEN in MCF-7/DOX and MDA-MB-231/DOX cells after the treatments of NC inhibitor and miR-3613-5p inhibitor. . Data are of 3 independent experiments.
(a)
(b)
(c)
(d)
(e)
(f)