Cancer-Associated Fibroblast-Derived Exosomal miRNA-320a Promotes Macrophage M2 Polarization In Vitro by Regulating PTEN/PI3Kγ Signaling in Pancreatic Cancer

Figure 1

CAFs-derived conditioned medium (CAFs-CM) facilitated macrophage M2 polarization. (a) qPCR analysis of mRNA expression of classic M2 signature markers (CD163, CD206, and IL-10) and classic M1 signature markers (HLA-DR and iNOS) in U937 cells cultured with HPDE6-C7-CM, PANC-CM, NF3-CM, NF6-CM, CAF3-CM, or CAF6-CM. (b) qPCR analysis of mRNA expression of CD163, CD206, and IL-10 in U937 cells cultured with NFs-CM, CAFs-CM, or CAFs/GW4869-CM. (c) Western blot analysis of CD163, CD206, and IL-10 protein expression in U937 cells cultured with NFs-CM, CAFs-CM, or CAFs/GW4869-CM. (d) Quantitative analysis of CD163, CD206, and IL-10 protein expression in U937 cells cultured with NFs-CM, CAFs-CM, or CAFs/GW4869-CM. (e) The effect of NFs-CM, CAFs-CM, or CAFs/GW4869-CM on proliferation of pancreatic cancer cells by CCK-8 assay. (f) The effect of NFs-CM, CAFs-CM, or CAFs/GW4869-CM on pancreatic cancer cells invasion was determined by the Transwell assay. Representative photographs (magnification, 100) and the number of invaded cells are displayed. . ^#vs CAFs-CM. qPCR, quantitative real-time PCR; CM, conditioned medium; NFs, normal fibroblasts; CAFs, cancer-associated fibroblasts.

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