Cancer-Associated Fibroblast-Derived Exosomal miRNA-320a Promotes Macrophage M2 Polarization In Vitro by Regulating PTEN/PI3Kγ Signaling in Pancreatic Cancer

Figure 2

CAFs-derived exosomes (CAFs-Exo) facilitated macrophage M2 polarization. (a) Electron micrograph analysis of exosomes collected from CAFs-CM (bar, 500 nm). (b) Size distribution and concentration range characterization of exosomes collected from CAFs-CM assayed with qNano. (c) Western blot analysis of protein expression of CD63, CD81, and HSP70 (markers of exosomes) in exosomes collected from CAFs-CM assayed. (d) qPCR analysis of CD163, CD206, and IL-10 mRNA expression in U937 cells cultured with NFs-Exo or CAFs-Exo. (e) Western blot analysis of protein expression of CD163, CD206, and IL-10 in U937 cells cultured with NFs-Exo or CAFs-Exo. (f) Quantitative analysis of CD163, CD206, and IL-10 protein expression in U937 cells cultured with NFs-Exo or CAFs-Exo. . qPCR, quantitative real-time PCR; CM, conditioned medium; NFs, normal fibroblasts; CAFs, cancer-associated fibroblasts; Exo, exosome.

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