Cancer-Associated Fibroblast-Derived Exosomal miRNA-320a Promotes Macrophage M2 Polarization In Vitro by Regulating PTEN/PI3Kγ Signaling in Pancreatic Cancer

Figure 3

Exosomes mediated the transfer of miRNA-320a from CAFs to macrophages. qPCR analysis of miR-106b, miR-148a, miR-125b, miR-320c, miR-320a, miR-1285, miR-422a, miR-29a, and miR-378d mRNA expression in NFs and CAFs (a) or NFs-Exo and CAFs-Exo (b). (c, d) qPCR analysis of miR-320a mRNA expression in 7 pancreatic cancer tissues-derived CAFs and 7 pancreatic cancer tissues-derived NFs or 7 pancreatic cancer tissues-derived CAFs-Exo and 7 pancreatic cancer tissues-derived NFs-Exo. (e–g) qPCR analysis of miR-320a mRNA expression in miRNA-320a-overexpressed CAFs (e), corresponding CAFs-Exo (f), and U937 cells after treatment with miRNA-320a-overexpressed CAFs-Exo (g). (h) Fluorescence microscope analysis of FAM-tagged miRNA-320a in U937cells treated with CAFs-Exo/FAM-miRNA-320a. . qPCR, quantitative real-time PCR; CM, conditioned medium; NFs, normal fibroblasts; CAFs, cancer-associated fibroblasts; Exo, exosome; CAFs-Exo/FAM-miRNA-320a, FAM-miRNA-320a-overexpressed CAFs-Exo.

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