Cancer-Associated Fibroblast-Derived Exosomal miRNA-320a Promotes Macrophage M2 Polarization In Vitro by Regulating PTEN/PI3Kγ Signaling in Pancreatic Cancer

Figure 4

miRNA-320a facilitated macrophage M2 polarization. qPCR analysis of classic M2 signature markers (CD163, CD206, and IL-10) and classic M1 signature markers (HLA-DR and iNOS) mRNA expression in U937 cells cultured with miRNA-320a mimics (a) or inhibitor (b). Western blot (c) and quantitative analysis (d) of CD163, CD206, and IL-10 protein expression in U937 cells cultured with miRNA-320a mimics. (e) The effect of U937/miR-320a-CM on pancreatic cancer cell proliferation by CCK-8 assay. (f, g) The effect of U937/miR-320a-CM on pancreatic cancer cells invasion was determined by the Transwell assay. Representative photographs (magnification, 100) and the number of invaded cells are displayed. . qPCR, quantitative real-time PCR; CM, conditioned medium; NFs, normal fibroblasts; CAFs, cancer-associated fibroblasts; U937/miR-320a-CM, miRNA-320a-overexpressed U937-derived CM.

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