Cancer-Associated Fibroblast-Derived Exosomal miRNA-320a Promotes Macrophage M2 Polarization In Vitro by Regulating PTEN/PI3Kγ Signaling in Pancreatic Cancer

Figure 6

miRNA-320a functions by targeting PTEN/PI3Kγ signaling. (a) Schematic representation of the miR-320a site in PTEN-3′UTR. (b, c) Luciferase activity was assayed in PCa cells co-transfected with miR-320a and luciferase reporters containing PTEN-3′UTR. Data are presented as the relative ratio of firefly luciferase activity to renilla luciferase activity. Western blot (d) and quantitative analysis (e) of PTEN, PI3Kγ, AKT, and p-AKT protein expression in miRNA-320a-overexpressed U937 cells. Western blot (f) and quantitative analysis (g) of CD163 and CD206 protein expression in U937 cells overexpressed with miRNA-320a in the presence or absence of PI3Kγ siRNA. . ^#vs miR-320a mimic group. qPCR, quantitative real-time PCR.

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