SH3BGRL Suppresses Liver Tumor Progression through Enhanced ATG5-Dependent Autophagy

Figure 1

SH3BGRL arrests liver cell cycle progression: (a) flow cytometry analysis of cell cycle progression of liver cancer cells LO2 and HepG2 with SH3BGRL overexpression, compared with the control cells (Vect). Cells were grown at 50%–70% of confluency and starved for 12 h, then cultured with the full medium in less than 12 h. (b) Immunoblots of endogenous SH3BGRL by knockdown with two specific shSH3BGRL-1 and -2. (c) Flow cytometry of the indicated pool cells with SH3BGRL knockdown (shSH3BGRL-1,2) as well as their scramble infection control cells (Scr) as in (a). (d) Immunoblots of the indicated proteins in cells with SH3BGRL knockdown. Cells were cultured in either nutrient-sufficient or deficient conditions for 14 hours, followed by immunoblotting analysis.

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