SH3BGRL Suppresses Liver Tumor Progression through Enhanced ATG5-Dependent Autophagy

Figure 2

SH3BGRL represses tumor formation of liver cells: (a) CCK-8 assay of the indicated cells with SH3BGRL knockdown under both nutrient-sufficient and deficient conditions. Data present as mean ± SD; n = 3; . (b, c) Xenograft tumor formation in nude mice by subcutaneous inoculation of 1 × 10⁶ LO2 cells (b) or HepG2 cells (c) with SH3BGRL overexpression, along with their parental control cells (GFP). Cells were injected into the flanks of nude mice. After 28 days, mice were sacrificed and the formed tumor weight was scored. Mean ± SD; n = 5; ; paired Student’s t-test. (d, e) Xenograft tumor formation in nude mice by inoculation of either LO2 cells (d) or HepG2 cells (e) with SH3BGRL knockdown and the scrambled control (Scr) as mentioned above. Mean ± SD; n = 5; ; unpaired Student’s t-test.

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