SH3BGRL Suppresses Liver Tumor Progression through Enhanced ATG5-Dependent Autophagy

Figure 3

SH3BGRL triggers basal autophagy of liver cells: (a, b) immunoblots of SH3BGRL and the autophagy-related proteins in cells with SH3BGRL overexpression (a) or endogenous SH3BGRL knockdown. β-actin was used as a loading control. GFP-vector (vector) and scrambled control (Scr) are shown. (c) Transmission electron microscopy of the formation of autophagosomes in LO2 and HepG2 cells with SH3BGRL overexpression. Enlarged images of the box are shown in the right panels. Representative images of double-membrane autophagosomes are pointed with arrows.

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