SH3BGRL Suppresses Liver Tumor Progression through Enhanced ATG5-Dependent Autophagy

Figure 4

SH3BGRL colocalizes with the ATG5-ATG12 complex: (a, b) mutual immunoprecipitation of SH3BGRL with ATG5 in both LO2 and HepG2 cells with stable overexpression of SH3BGRL (a) or the parental normal cells with SH3BGRL and ATG5 antibodies, respectively. ATG5 and ATG12 were detected at their covalent complex position to indicate autophagy formation. (c) Immunofluorescence of the colocalization SH3BGRL with ATG5 in both LO2 and HepG2 cells with stable overexpression of SH3BGRL or the parental normal cells (GFP) with SH3BGRL and ATG5 antibodies, respectively. LC3 was stained to show its puncta to indicate the autophagy formation.

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