SH3BGRL Suppresses Liver Tumor Progression through Enhanced ATG5-Dependent Autophagy

Figure 5

SH3BGRL stabilizes ATG5 from ubiquitination-mediated degradation: (a, b) immunoblots of ATG5 and ATG12 in LO2 cells with SH3BGRL overexpression or knockdown (a) or in HepG2 cells overexpressing SH3BGRL (b) with CHX or Act.D treatments. (c) Ubiquitination of ATG5 analysis by ATG5 antibody immunoprecipitation and immunoblotting in SH3BGRL-overexpressing HepG2 cells. MG132 was used to block ATG5 proteasome degradation.

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