The Effects of circ_000558/miR-1225-5p/ARL4C on Regulating the Proliferation of Renal Cell Carcinoma Cells

Figure 3

circ_000558 regulated RCC cell proliferation by inhibiting miR-1225-5p. (a) Venn diagram showed the overlapped potential target miRNAs in GSE23085, GSE16441, circBank, and circular RNA interactome. The binding site between miR-1225-5p and circ_000558 was shown. (b) The expression of miR-1225-5p in renal carcinoma cells (ACHN, 769-P, and Caki-1) and human renal epithelial cells (HK2) was tested by qPCR analysis. (c) The expression of miR-1225-5p in 769-P and ACHN cells after control and circ_000558 overexpression plasmid transfection was measured by qPCR. (d) The relative luciferase intensity in 769-P and ACHN cells after wild-type of circ_000558 (circ-WT) or mutation type of circ_000558 (circ-Mut) and miR-1225-5p mimics (miR-mimics) cotransfect was tested by dual-luciferase reporter assay. (e) The cell viability of 769-P and ACHN cells after miR-mimics, miR-inhibitor, or miR-mimics + ov-circ transfection was tested by CCK-8 assay. (f) The cell apoptosis of 769-P and ACHN cells after miR-mimics, miR-inhibitor, or miR-mimics + ov-circ transfection was tested by flow cytometry. ( < 0.01;  < 0.001).

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