Research Article

Tumor Suppressor miR-613 Alleviates Non-Small Cell Lung Cancer Cell via Repressing M2 Macrophage Polarization

Figure 4

miR-613 regulates the M2 macrophage polarization. After differentiating THP-1 cells into macrophages with 24 h treatment of 150 nM phorbol 12-myristate 13-acetate, the cells were fostered for 48 h with 20 ng/mL IL-4 to achieve the M2 macrophages, and miR-613 mimic was transfected into M2 macrophages. (a) The expressions of Arg-1 and CD206 (markers for M2 macrophages) and iNOS and CD86 (markers for M1 macrophages) were measured using qRT-PCR. (b) The IL-10 and TGF-β levels in cell culture supernatant were tested by enzyme-linked immunosorbent assay (ELISA). vs. NC-mimic. , , vs. IL-4+NC-mimic.
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