Tumor Suppressor miR-613 Alleviates Non-Small Cell Lung Cancer Cell via Repressing M2 Macrophage Polarization

Figure 5

miR-613 regulates NSCLC cell growth by repressing M2 macrophage polarization. Macrophages with different treatments (as in Figure 4) were co-cultured with H1650 cells. H1650 cells were grouped: H1650+M^NCmimic, H1650+M^IL-4+NCmimic and H1650+M^{IL-4+miR-613mimic}. (a) The schematic diagram of the co-culture system. (b) CCK-8 was conducted to assess H1650 cell viability. (c) Evaluation of H1650 cell proliferation by the Edu method (scale bar: 100 μm). (d) Detection of H1650 cell apoptosis by flow cytometry. (e and f) H1650 cell migration was tested using Transwell (scale bar: 200 μm) and wound-healing analyses (scale bar: 400 μm). (g) H1650 cell invasion was measured by Transwell (scale bar: 200 μm). vs. H1650+M^NCmimic. , vs. H1650+M^IL-4+NCmimic.

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